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Single-molecuul Förster Resonance Energy Transfer methoden voor real-time onderzoek van de Holliday Junction-resolutie door GEN1

JoVE Journal
Genetica

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JoVE Journal Genetica

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

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Single-molecuul Förster Resonance Energy Transfer methoden voor real-time onderzoek van de Holliday Junction-resolutie door GEN1

8,955 Views

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11:27 min

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September 18, 2019

DOI:

10.3791/60045-v

DOI:

10.3791/60045-v

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11:27 min

September 18, 2019

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2 Views

Mohamed A. Sobhy*¹, Amer Bralić*¹, Vlad-Stefan Raducanu, Muhammad Tehseen, Yujing Ouyang, Masateru Takahashi, Fahad Rashid, Manal S. Zaher, Samir M. Hamdan

¹Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)

Tags

Single-molecule FÃ¶rster Resonance Energy Transfer (FRET)Holliday Junction GEN1 Real-time Investigation Enzymatic Systems Single-molecule Methods Biomolecular Interactions Optical And Force Measurements Single-channel Flow Cell Quartz Slide Double Adhesive Sheet Polytetrafluoroethylene Tweezers Polyethylene Tubing Epoxy Glue Avidin PBS

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