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一种可定制的酶化生产与纯化天然产物的方法
JoVE Journal
Biochimica
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JoVE Journal Biochimica
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
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一种可定制的酶化生产与纯化天然产物的方法

9,321 Views

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07:59 min

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October 04, 2019

DOI:

10.3791/59992-v

DOI:
10.3791/59992-v

•

07:59 min
October 04, 2019

•

12 Views
, , , , ,
1Department of Plant Biology,University of California Davis

Summary

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在这里，我们提出了易于使用的协议，通过大肠杆菌或尼科蒂亚纳本塔米亚中的生物合成酶的组合表达来生产和纯化二苯基代谢物，然后是色谱产物净化。由此产生的代谢物适用于各种研究，包括分子结构表征、酶功能研究和生物活性测定。

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Cite this Article

Murphy, K. M., Chung, S., Fogla, S., Minsky, H. B., Zhu, K. Y., Zerbe, P. A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products. J. Vis. Exp. (152), e59992, doi:10.3791/59992 (2019).

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