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斑马鱼原位杂交染色的基因分型与定量
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
DOI:

05:41 min

January 28, 2020

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Capitoli

  • 00:04Titolo
  • 00:40ISH-stained Embryo Imaging and DNA Extraction
  • 02:26Image Analysis with Fiji Software
  • 03:53Results: Parallel Image Quantitation and Genotyping Protocol
  • 05:13Conclusion

Summary

Traduzione automatica

基因编辑技术使研究人员能够相对轻松地生成斑马鱼突变体来研究基因功能。在这里,我们提供了一个指南,以执行平行胚胎基因分型和定量的原位杂交信号在斑马鱼。这种无偏的方法在基于原位杂交的表型分析中提供了更高的精度。

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