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Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

DOI:
10.3791/59920-v

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06:03 min

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July 15, 2019

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, , ,
1Department of Molecular Biosciences,University of Texas at Austin, 2School of Pharmacy, College of Pharmacy,Kaohsiung Medical University, 3Graduate Institute of Medical Sciences, College of Medicine,Taipei Medical University, 4Department of Microbiology and Immunology,Dalhousie University, 5Department of Microbiology and Immunology, School of Medicine, College of Medicine,Taipei Medical University

Capitoli

  • 00:04Titolo
  • 00:27Time-of-Drug-Addition
  • 02:25Molecular Docking Analysis
  • 04:06Results: Representative Effects of Small Molecule (CHLA and PUG) Binding Against CVA16 Infectivity
  • 05:33Conclusion

Summary

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The goal of the protocol is to illustrate the different assays relating to viral entry that can be used to identify candidate viral entry inhibitors.

Tags

Viral Entry AssayMolecular DockingAntiviral CandidatesCoxsackievirus A16RD CellsPlaque-forming UnitsMethylcelluloseCrystal VioletPubChemProtein Data BankMolecular ProgramBio-computing Program

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