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人类干细胞衍生的伊拉尔肠细胞单层中M细胞样细胞的诱导分化
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
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人类干细胞衍生的伊拉尔肠细胞单层中M细胞样细胞的诱导分化

8,198 Views

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11:34 min

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July 26, 2019

DOI:

10.3791/59894-v

DOI:
10.3791/59894-v

•

11:34 min
July 26, 2019

•

6 Views
, , ,
1Program in Immunology,Sackler School of Graduate Biomedical Sciences, 2Department of Molecular Virology and Microbiology,Baylor College of Medicine, 3Department of Molecular Biology and Microbiology,Tufts University

Summary

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该协议描述了如何诱导M细胞在人类干细胞衍生的卵单层中的分化,以及评估其发育的方法。

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M CellsIntestinal Stem CellsInduced DifferentiationIleal Enteroid MonolayersExtracellular MatrixTranswell MembranesEDTATrypsinCell DisaggregationSingle Cells

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Cite this Article

Fasciano, A. C., Blutt, S. E., Estes, M. K., Mecsas, J. Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers. J. Vis. Exp. (149), e59894, doi:10.3791/59894 (2019).

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