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从人类唾液腺中分离唾液表皮细胞,作为脂质层或单层体进行体外生长
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
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从人类唾液腺中分离唾液表皮细胞,作为脂质层或单层体进行体外生长

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08:03 min

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July 15, 2019

DOI:

10.3791/59868-v

DOI:
10.3791/59868-v

•

08:03 min
July 15, 2019

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4 Views
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1Department of Molecular Genetics, Biochemistry, and Microbiology,University of Cincinnati College of Medicine

Tags

Salivary Epithelial CellsPrimary Cell CultureSalispheresOrganoidSalivary GlandDispaseCollagenaseCell IsolationCell CultureCell Strainer

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Beucler, M. J., Miller, W. E. Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers. J. Vis. Exp. (149), e59868, doi:10.3791/59868 (2019).

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