Rivista
/
Immunologia e infezioni
/
Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

DOI:
10.3791/59868-v

•

08:03 min

•

July 15, 2019

•

,
1Department of Molecular Genetics, Biochemistry, and Microbiology,University of Cincinnati College of Medicine

Capitoli

  • 00:04Titolo
  • 00:54Tissue Digestion and Well Coating with BMM (Basement Membrane Matrix)
  • 02:07Filtering of Undigested Tissue, RBC Lysis, and Cell Plating
  • 03:53Subculturing the Salispheres and Maintenance on BMM
  • 05:23Subculturing the Salispheres on Treated Plastic Tissue Culture Dishes
  • 06:43Results: Primary Human Salisphere Cells Cultured as Spheres or as a Monolayer
  • 07:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present a method for isolating and cultivating primary human salivary gland-derived epithelial cells. These cells exhibit gene expression patterns consistent with them being of salivary epithelial origin and can be grown as salispheres on basement membrane matrices derived from Engelbreth-Holm-Swarm tumor cells or as monolayers on treated culture dishes.

Tags

Salivary Epithelial CellsPrimary Cell CultureSalispheresOrganoidSalivary GlandDispaseCollagenaseCell IsolationCell CultureCell Strainer

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

A Method for the Measurement of Salivary Gland Function in Mice

Read Article