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环境细颗粒物小鼠组织中三种 DNA 损伤的质谱定量及水平评价
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
DOI:

12:15 min

May 29, 2019

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Capitoli

  • 00:04Titolo
  • 01:20DNA Extraction
  • 04:18Quantification by HPLC-UV and Solid Phase Extraction for Analyses
  • 06:20Quantification by HPLC-ESI-MS/MS
  • 09:19Quantification of the DNA Lesions
  • 10:36Results: HPLC-UV and HPLC-ESI-MS/MS Analyses
  • 11:25Conclusion

Summary

Traduzione automatica

我们在这里描述的方法敏感和准确定量的病变 8-氧-7-二氢-2 '-脱氧鸟苷 (8-氧 ',1, n6-乙基 2 '-脱氧腺苷 (1,n6-do)和 1,n2-在 DNA 中的乙二-2 '-脱氧鸟苷 (1,n2-dguo)。应用该方法对暴露的阿可姆小鼠组织 (肺、肝、肾) 环境细颗粒物 (PM2.5) 的影响进行了评价。

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