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Journal / Bioengineering / Standardized and Scalable Assay to Study Perfused …
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Bioengineering

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JoVE Journal Bioengineering
Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
 

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Article DOI: 10.3791/59678-v • 10:47 min • November 6th, 2019
November 6th, 2019 •
1,2, 1, 3, 3, 4, 5, 2, 1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas

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This method describes the culture of iPSC-derived endothelial cells as 40 perfused 3D microvessels in a standardized microfluidic platform. This platform enables the study of gradient-driven angiogenic sprouting in 3D, including anastomosis and stabilization of the angiogenic sprouts in a scalable and high-throughput manner.

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Standardized Scalable Assay Study Perfused 3D Angiogenic Sprouting IPSC-derived Endothelial Cells In Vitro New Targets Angiogenesis Assay Cellular Micro Environments Gradients Perfusion High Throughput Screening Health Disease Cancers Tissues Repair Cardiovascular Diseases Tissue Regeneration Microfluidic Platform Law Of Volumes Pipette Microscope Plate Upside Down Microfluidic Channels Hold The Plate Wendy Stam Technician Transfer Microfluidic 384-well Plate Sterile Laminar Flow Hood
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