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生体内での2光子蛍光寿命イメージング顕微鏡を用いて頭固定振る舞いマウスにおけるタンパク質キナーゼの活動を可視化する
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
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生体内での2光子蛍光寿命イメージング顕微鏡を用いて頭固定振る舞いマウスにおけるタンパク質キナーゼの活動を可視化する

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10:41 min

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June 07, 2019

DOI:

10.3791/59526-v

DOI:
10.3791/59526-v

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10:41 min
June 07, 2019

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1 Views
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1Vollum Institute,Oregon Health & Science University

Tags

Protein Kinase APKATAKARαTwo-photon Fluorescence Lifetime Imaging Microscopy2pFLIMNeuromodulationCAMPFRETHead-fixed BehaviorIn Vivo ImagingMotor Cortex

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Cite this Article

Jongbloets, B. C., Ma, L., Mao, T., Zhong, H. Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (148), e59526, doi:10.3791/59526 (2019).

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