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In tempo reale Live-cella flusso Cytometry per indagare l'afflusso del calcio, la formazione del poro e fagocitosi dai recettori P2X7 in cellule progenitrici neurali adulto
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1Griffith Institute for Drug Discovery,Griffith University, 2Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science,University of Sydney, 4Bosch Institute,University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit,St. Vincent’s Centre for Applied Medical Research, 6School of Medical Sciences,The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science,Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health,University of Melbourne
Capitoli
- 00:04Titolo
- 00:58Preparation of a Single-cell Suspension of Neural Progenitor Cells for Analysis by Flow Cytometry
- 02:11Measuring Calcium Influx by Live-cell Flow Cytometry
- 05:21Measuring Pore Formation by Live-cell Flow Cytometry
- 07:02Measuring Phagocytosis by Live-cell Flow Cytometry
- 09:02Results: Analyses of Calcium Influx, Pore Formation, and Phagocytosis by Live-cell Flow Cytometry
- 10:46Conclusion
Fornire sensibilità unicellulare, citometria a flusso in tempo reale è in grado di quantificare le funzioni del ricevitore multimodale di culture vivono. Utilizzando cellule progenitrici neurali adulte, la funzione del recettore P2X7 è stata valutata tramite afflusso del calcio rilevato dal colorante indicatore di calcio, la formazione dei pori transmembrana dall'assorbimento di bromuro di etidio e fagocitosi utilizzando granuli di lattice fluorescente.
