JoVE
JoVE
Sportello unico per docenti
Ricerca
Comportamento
Biochimica
Biologia
Bioingegneria
Ricerca sul cancro
Chimica
Biologia dello sviluppo
Ingegneria
Ambiente
Genetica
Immunologia e infezioni
Medicina
Neuroscienze
JoVE Journal
Enciclopedia JoVE degli Esperimenti
JoVE Chrome Extension
Didattica
Biologia
Chimica
Clinical
Ingegneria
Scienze ambientali
Pharmacology
Fisica
Psicologia
Statistica
JoVE Core
JoVE Educazione Scientific
Manuale di laboratorio JoVE
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Autori
Personale delle biblioteche
Scuole superiori
Chi siamo
Sign-In
Accedi
Contattaci
Ricerca
JoVE Journal
Enciclopedia JoVE degli Esperimenti
Didattica
JoVE Core
JoVE Educazione Scientific
Manuale di laboratorio JoVE
Scuole superiori
IT
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
IT
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Ricerca
Comportamento
Biochimica
Bioingegneria
Biologia
Ricerca sul cancro
Chimica
Biologia dello sviluppo
Ingegneria
Ambiente
Genetica
Immunologia e infezioni
Medicina
Neuroscienze
Products
JoVE Journal
Enciclopedia JoVE degli Esperimenti
Didattica
Biologia
Chimica
Clinical
Ingegneria
Scienze ambientali
Pharmacology
Fisica
Psicologia
Statistica
Products
JoVE Core
JoVE Educazione Scientific
Manuale di laboratorio JoVE
JoVE Quiz
JoVE Business
Video consigliati per il tuo insegnamento
Teacher Resources
Get in Touch
Instant Trial
Log In
IT
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Rivista
/
Biologia dello sviluppo
/
P2X7 受体实时活细胞流式细胞仪检测成人神经祖细胞中的钙影响、毛孔形成和吞噬作用
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal
Biologia dello sviluppo
Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
Please note that all translations are automatically generated.
Click here for the English version.
P2X7 受体实时活细胞流式细胞仪检测成人神经祖细胞中的钙影响、毛孔形成和吞噬作用
DOI:
10.3791/59313-v
•
11:47 min
•
April 03, 2019
•
Hannah C. Leeson
2
,
Tailoi Chan-Ling
4
,
Michael D. Lovelace
5,6
,
Jeremy D. Brownlie
,
Michael W. Weible II*
1,4,7
,
Ben J. Gu*
8
1
Griffith Institute for Drug Discovery
,
Griffith University
,
2
Australian Institute for Bioengineering and Nanotechnology
,
University of Queensland
,
3
Discipline of Anatomy and Histology, School of Medical Science
,
University of Sydney
,
4
Bosch Institute
,
University of Sydney
,
5
Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit
,
St. Vincent’s Centre for Applied Medical Research
,
6
School of Medical Sciences
,
The University of New South Wales (UNSW) Medicine, Sydney, New South Wales
,
7
School of Environment and Science
,
Griffith University, Brisbane, Queensland
,
8
Florey Institute of Neuroscience and Mental Health
,
University of Melbourne
Capitoli
00:04
Titolo
00:58
Preparation of a Single-cell Suspension of Neural Progenitor Cells for Analysis by Flow Cytometry
02:11
Measuring Calcium Influx by Live-cell Flow Cytometry
05:21
Measuring Pore Formation by Live-cell Flow Cytometry
07:02
Measuring Phagocytosis by Live-cell Flow Cytometry
09:02
Results: Analyses of Calcium Influx, Pore Formation, and Phagocytosis by Live-cell Flow Cytometry
10:46
Conclusion
Summary
Traduzione automatica
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Traduzione automatica
提供单细胞灵敏度, 实时流式细胞仪是唯一适合量化多模态受体功能的活培养物。利用成体神经祖细胞, 通过钙指示剂检测钙流入、溴化乙酯吸收的跨膜孔形成和荧光乳胶珠的吞噬作用来评估 P2X7 受体功能。
Tags
Real-time Live-cell Flow Cytometry
Calcium Influx
Pore Formation
Phagocytosis
P2X7 Receptors
Neural Progenitor Cells
Subventricular Zone
Hippocampus
Neurospheres
Cell Culture
Calcium Indicator Dye
Calcium-free Sodium Medium
Potassium Medium
Article
Embed
AGGIUNGI ALLA PLAYLIST
Usage Statistics
Video correlati
人类神经干细胞从外周血造血祖细胞直接诱导
从脑室周围地区的成年大鼠和人类脊髓神经分离干/祖细胞
步特定老鼠的精子细胞分选流式细胞仪
成年转基因斑马鱼视网膜外植体培养由多光子显微镜活细胞成像
隔离并通过流式细胞仪分析胰腺间充质细胞
通过机械组织分离和流式细胞术对哺乳动物雄性生殖细胞多特异性纯化的标准化方法
多光子时间推移成像实时可视化发展: 迁移神经嵴细胞在斑马鱼胚胎中的可视化
应用造血干细胞移植评估骨髓增生异常综合征的起源
成年小鼠数字截肢和再生:研究哺乳动物暴发体形成和内膜性细胞化的简单模型
评估原小鼠眼部表面细胞/干细胞对紫外线-C (UV-C) 损伤的反应
Read Article