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Lentivirale Vector Platform voor de efficiënte levering van Epigenome-bewerkingsgereedschap in mens geïnduceerde pluripotente stamcel afkomstige ziekte modellen
JoVE Journal
Genetica
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JoVE Journal Genetica
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
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Lentivirale Vector Platform voor de efficiënte levering van Epigenome-bewerkingsgereedschap in mens geïnduceerde pluripotente stamcel afkomstige ziekte modellen

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13:47 min

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March 29, 2019

DOI:

10.3791/59241-v

DOI:
10.3791/59241-v

•

13:47 min
March 29, 2019

•

6 Views
, , , , , , , ,
1Department of Neurology,Duke University Medical Center, 2Center for Genomic and Computational Biology,Duke University Medical Center, 3Viral Vector Core,Duke University Medical Center, 4Division of Gynecologic Oncology, Department of Obstetrics and Gynecology,Duke University Medical Center

Summary

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Gerichte DNA epigenome bewerken vertegenwoordigt een krachtige therapeutische aanpak. Dit protocol beschrijft de productie, reiniging en concentratie van de alles-in-één lentivirale vectoren herbergen van de CRISPR-dCas9-DNMT3A-transgenic voor bewerken van epigenome's in menselijke geïnduceerde pluripotente stamcel (hiPSC)-afgeleid van neuronen.

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Cite this Article

Tagliafierro, L., Ilich, E., Moncalvo, M., Gu, J., Sriskanda, A., Grenier, C., Murphy, S. K., Chiba-Falek, O., Kantor, B. Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models. J. Vis. Exp. (145), e59241, doi:10.3791/59241 (2019).

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