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诱导BALB/c小鼠药物诱导的自身免疫性肝炎研究其致病机制
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
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诱导BALB/c小鼠药物诱导的自身免疫性肝炎研究其致病机制

DOI:
10.3791/59174-v

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11:36 min

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May 29, 2020

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, , , ,
1Department of Anesthesiology and Critical Care Medicine,Johns Hopkins University, 2Department of Pediatrics and Pathology,Johns Hopkins University

Capitoli

  • 00:04Introduction
  • 02:04Isolation of the S-100 Fraction of the Liver
  • 03:36Trifluoroacetylation of S-100
  • 05:45Determination of CD4+ T cell Immune Responses
  • 07:26Isolation of Infiltrating Immune Cells from Immunized Mice
  • 09:27Results: Cell Immune Responses
  • 10:45Conclusion

Summary

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我们描述了BALB / c小鼠中的体内免疫，转化性肝炎模型，可用于研究药物诱导的自身免疫性肝炎的发病机制，包括该疾病中看到的性别差异。我们将描述该模型如何使用体内和体外实验技术演示可重复的分析。

Tags

Drug-induced HepatitisAutoimmune HepatitisBALB/c MiceLiver DiseaseCirrhosisIdiosyncratic DILIImmune-mediated HepatitisAnesthetic HepatitisViral HepatitisFibrosisDiagnostic TestsPathogenic MechanismsMouse ModelLiver PerfusionLiver Homogenization

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