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在生物安全2级环境中制备假型颗粒研究高致病性冠状病毒
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08:40 min
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March 01, 2019
DOI:
1Department of Microbiology and Immunology, College of Veterinary Medicine,Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering,Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences,Cornell University, 5Horae Gene Therapy Center,University of Massachusetts Medical School
Summary
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在这里, 我们提出了一个协议, 以产生伪型粒子在 bsl-2 设置纳入高致病性病毒中东呼吸综合征和严重急性呼吸综合征冠状病毒的尖峰蛋白。这些伪类型粒子包含一个荧光素酶报告基因, 允许病毒进入目标宿主细胞的定量。
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Cite this Article
Millet, J. K., Tang, T., Nathan, L., Jaimes, J. A., Hsu, H., Daniel, S., Whittaker, G. R. Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting. J. Vis. Exp. (145), e59010, doi:10.3791/59010 (2019).
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