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Immunologia e infezioni

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基于多色流式细胞仪的 t 细胞线粒体和溶酶体的定量

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

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基于多色流式细胞仪的 t 细胞线粒体和溶酶体的定量

12,577 Views

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06:22 min

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January 09, 2019

DOI:

10.3791/58844-v

DOI:

10.3791/58844-v

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06:22 min

January 09, 2019

•

10 Views

Chin-Wen Wei¹, Tyng-An Zhou¹, Ivan L. Dzhagalov¹, Chia-Lin Hsu¹

¹Institute of Microbiology and Immunology,National Yang-Ming University

Summary

本文说明了一种对活细胞中线粒体或溶酶体进行量化的有力方法。将溶酶体或线粒体特异性染料与针对表面标记物的荧光共轭抗体结合使用, 可以通过使用多色流式细胞术。

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