Rivista
/
Biologia
/
一种研究受体配体相互作用的 bw 报告系统
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
A BW Reporter System for Studying Receptor-Ligand Interactions
Please note that all translations are automatically generated. Click here for the English version.

一种研究受体配体相互作用的 bw 报告系统

DOI:
10.3791/58685-v

•

06:05 min

•

January 07, 2019

•

, , ,
1Department of Hematology,Hadassah – Hebrew University Medical Center, 2The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC),Hadassah – Hebrew University Medical Center

Capitoli

  • 00:04Titolo
  • 00:36Transfection of the Plasmid that Expresses the Chimeric Protein into BW Cells
  • 03:10Incubation of the Transfected BW Cells with Targets
  • 03:32ELISA
  • 04:38Results: BW Reporter System Demonstrated
  • 05:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了如何建立一个可用于识别和量化受体配体相互作用的记者系统。

Tags

BW Reporter SystemReceptor-ligand InteractionsLigandEndogenous LigandTransfectionPcDNA3 PlasmidSodium AcetateEthanol PrecipitationElectroporationG418 Selection96-well PlateELISA

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

研究与基于荧光素酶的记者基因检测膜生源

基于分体式泛素膜酵母双杂交（神话）系统：一个强大的工具用于识别蛋白质 - 蛋白质相互作用

确定远距离的DNA相互作用的相关染色体陷阱

使用网关兼容双分子荧光互补（附设）系统在植物蛋白相互作用检测

基于亲和力，外的相互作用筛选（AVEXIS）外低亲和力受体 - 配体相互作用的可扩展检测

对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统

研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移

RNA催化剂作为记者为筛选药物对RNA编辑在锥虫

转染RAW264.7细胞用荧光素酶报告基因

在拟南芥原生质体转染昼夜的表型有荧光时钟记者的快速分析

Read Article