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优化干扰素伽隆弹性体检测在几内亚猪模型接种疫苗后 t 细胞反应
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
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优化干扰素伽隆弹性体检测在几内亚猪模型接种疫苗后 t 细胞反应

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08:13 min

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January 20, 2019

DOI:

10.3791/58595-v

DOI:
10.3791/58595-v

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08:13 min
January 20, 2019

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2 Views
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1Inovio Pharmaceuticals, Inc, 2Mycotic and Parasitic Agents and Mycobacteria,Robert Koch Institute

Tags

ELISpot AssayT Cell ResponsesGuinea Pig ModelVaccinationImmunotherapeuticsHumoral ImmunityPeripheral BloodKineticsCapture Anti-guinea Pig Interferon Gamma Antibody VE4Density Gradient MediumPBMCs

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Schultheis, K., Schaefer, H., Pugh, H. M., Yung, B. S., Oh, J., Nguyen, J., Humeau, L., Broderick, K. E., Smith, T. R. Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination. J. Vis. Exp. (143), e58595, doi:10.3791/58595 (2019).

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