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Journal / Immunology and Infection / Purification of Extracellular Trypanosomes,…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
 

Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

Article DOI: 10.3791/58415-v • 14:26 min • April 6th, 2019
April 6th, 2019 •
1,2, 1,2, 1,2, 3, 1,2,4, 1,2, 3, 3, 1,2,4
1Univ. Bordeaux UMR INTERTRYP 177 IRD CIRAD, 2IRD UMR 177 INTERTRYP CIRAD, 3CNRS, UMR 5234, Microbiologie Fondamentale et Pathogénicité, Univ. Bordeaux, 4Centre Hospitalier Université Bordeaux

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This method of trypanosome separation from blood depends on their surface charge being less negative than mammalian blood cells. Infected blood is placed and treated on an anion-exchanger column. This method, the most fitting diagnostic for African trypanosomiasis, provides purified parasites for immunological, biological, biochemical, pharmaceutical and molecular biology investigations.

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Purification Extracellular Trypanosomes African Trypanosomes Blood Anion-exchangers Diethylaminoethyl-cellulose Columns Sleeping Sickness Animal African Trypanosomiasis Tsetse Fly Transmitted Protist Parasites Serology Diagnosis Treatment Follow-up Parasite Concentration Techniques DEAE Cellulose Method Visualizing Isolating Parasites Field Conditions Immunological Investigations Biological Investigations Biochemical Investigations Pharmaceutical Investigations Molecular Biological Investigations
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