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Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster
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JoVE Journal Neuroscienze
Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster

Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster

DOI:
10.3791/58375-v

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06:45 min

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November 21, 2018

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, , ,
1Department of Entomology,Louisiana State University AgCenter, 2Department of Entomology,Virginia Tech, 3Department of Entomology and Nematology, Emerging Pathogens Institute,University of Florida

Capitoli

  • 00:04Titolo
  • 00:35Preparation of Software for Dissection and Recording
  • 02:05Dissect and Prepare the Larval Drosophila CNS
  • 03:35Extracellular Recording of Drosophila CNS
  • 05:22Results: Neurophysiological Recordings from the CNS of Third-Instar Larvae of D. melanogaster
  • 05:59Conclusion

Summary

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This protocol describes a method to record the descending electrical activity of the Drosophila melanogaster central nervous system to enable the cost-efficient and convenient testing of pharmacological agents, genetic mutations of neural proteins, and/or the role of unexplored physiological pathways.

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Keywords: Electrophysiological RecordingCentral Nervous SystemDrosophila MelanogasterInsect ToxicologyInsect PhysiologyElectrogenesisInsecticide Modes Of ActionNervous SystemAcquisition/analysis SoftwareRaw SignalAmplifierEvent CountingThresholdRate PlotHertzDissectionThird-instar LarvaeSalineForcepsCentral Nervous System Extraction

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