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用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板
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November 06, 2018
DOI:
1Graduate Program in Pharmacology and Experimental Therapeutics,University of Cologne, 2Department of Dermatology and Venereology,University Hospital of Cologne, 3Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne, 4Department of General Ecology, Institute for Zoology, Biocenter Cologne,University of Cologne
Summary
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节点和脊索板是小鼠胚胎发育过程中的瞬态信号组织者, 可以使用多种技术进行可视化。在这里, 我们详细地描述了如何执行两种技术来研究它们的结构和形态发生: 1) 扫描电子显微镜 (sem);和 2) 全安装免疫荧光 (wmif)。
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Xiao, C., Nitsche, F., Bazzi, H. Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence. J. Vis. Exp. (141), e58321, doi:10.3791/58321 (2018).
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