È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Institute of Vegetables,Zhejiang Academy of Agricultural Sciences, 2State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease,Zhejiang Academy of Agricultural Sciences, 3Institute of Horticulture,Zhejiang Academy of Agricultural Sciences, 4Shanghai Biozeron Biotechnology Co., Ltd
Capitoli
- 00:04Titolo
- 01:19Seed Sterilization and Germination
- 02:09Seedling Growth and Grafting
- 02:48Post-grafting Management, Cold Treatment, and Sampling
- 03:45Library Preparation and High-throughput Sequencing
- 05:20Results: miRNA Up- and Down-regulated in Cold Treated Homografts and Heterografts
- 06:17Conclusion
Nous présentons ici un protocole détaillé pour faire efficacement des homo - et xénogreffe entre pastèque et gourde, en plus des méthodes de prélèvement d’échantillons de tissus, la génération de données et l’analyse des données, pour l’étude des microARN froid-sensible.
