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Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
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Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

DOI:
10.3791/58242-v

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07:22 min

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November 20, 2018

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, , , , , ,
1Institute of Vegetables,Zhejiang Academy of Agricultural Sciences, 2State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease,Zhejiang Academy of Agricultural Sciences, 3Institute of Horticulture,Zhejiang Academy of Agricultural Sciences, 4Shanghai Biozeron Biotechnology Co., Ltd

Capitoli

  • 00:04Titolo
  • 01:19Seed Sterilization and Germination
  • 02:09Seedling Growth and Grafting
  • 02:48Post-grafting Management, Cold Treatment, and Sampling
  • 03:45Library Preparation and High-throughput Sequencing
  • 05:20Results: miRNA Up- and Down-regulated in Cold Treated Homografts and Heterografts
  • 06:17Conclusion

Summary

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Here we present a detailed protocol for efficiently making homo- and heterografts between watermelon and bottle gourd, in addition to methods of tissue sampling, data generation, and data analysis, for the investigation of cold-responsive microRNAs.

Tags

Keywords: WatermelonBottle GourdGraftingHomo- And HeterograftsCold StressMiRNA ResponsePlant Grafting TechniqueSeed GerminationSeedling PreparationGrafting ProcedureGrowth ChamberHumidity Control

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