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Isolering av netthinnen arterioler for Ex Vivo celle fysiologi studier
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Biologia
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JoVE Journal Biologia
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies

Isolering av netthinnen arterioler for Ex Vivo celle fysiologi studier

DOI:
10.3791/57944-v

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12:42 min

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July 14, 2018

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, , , , , , , ,
1Centre for Experimental Medicine,Queen’s University of Belfast, 2Centre for Biomedical Sciences (Education),Queen’s University of Belfast, 3Department of Pharmaceutical Chemistry and Pharmacognosy,Naresuan University, 4School of Medicine, Dentistry and Biomedical Sciences,Queen’s University of Belfast

Capitoli

  • 00:00Titolo
  • 01:23Isolation of Retinal Arterioles
  • 05:09Arteriolar Pressure Myography
  • 07:22Patch-Clamp Electrophysiology
  • 09:58Risultati
  • 12:00Conclusion

Summary

Traduzione automatica

Traduzione automatica

Dette manuskriptet beskriver en enkel protokoll for isolering av arterioler fra rotte netthinnen som kan brukes i elektrofysiologiske, kalsium bildebehandling og trykk myography studier.

Tags

Keywords: Retinal ArteriolesRetinal Blood FlowRetinal PerfusionVascular Smooth MusclePatch ClampCalcium ImagingPressure MyographyRetinal DiseasesDiabetic RetinopathyGlaucomaBranch Vein OcclusionsEye DissectionRetina IsolationLow Calcium Hanks’ Solution

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