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体外细胞生理学研究中视网膜微动脉的分离
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Biologia
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JoVE Journal Biologia
Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies
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体外细胞生理学研究中视网膜微动脉的分离

DOI:
10.3791/57944-v

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12:42 min

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July 14, 2018

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, , , , , , , ,
1Centre for Experimental Medicine,Queen’s University of Belfast, 2Centre for Biomedical Sciences (Education),Queen’s University of Belfast, 3Department of Pharmaceutical Chemistry and Pharmacognosy,Naresuan University, 4School of Medicine, Dentistry and Biomedical Sciences,Queen’s University of Belfast

Capitoli

  • 00:00Titolo
  • 01:23Isolation of Retinal Arterioles
  • 05:09Arteriolar Pressure Myography
  • 07:22Patch-Clamp Electrophysiology
  • 09:58Risultati
  • 12:00Conclusion

Summary

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这篇手稿描述了一个简单的协议, 从大鼠视网膜分离的小动脉, 可用于电生理, 钙成像和压力 myography 研究。

Tags

Keywords: Retinal ArteriolesRetinal Blood FlowRetinal PerfusionVascular Smooth MusclePatch ClampCalcium ImagingPressure MyographyRetinal DiseasesDiabetic RetinopathyGlaucomaBranch Vein OcclusionsEye DissectionRetina IsolationLow Calcium Hanks’ Solution

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