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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
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Biochimica
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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

8,986 Views

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09:30 min

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August 06, 2018

DOI:

10.3791/57902-v

DOI:
10.3791/57902-v

•

09:30 min
August 06, 2018

•

8 Views
, , , , ,
1Department of Biochemistry and Molecular Biology,University of Kansas Medical Center, 2Department of Chemistry,Massachusetts Institute of Technology

Summary

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Here we present a protocol to monitor the assembly and disassembly of the anthrax toxin using biolayer interferometry (BLI). Following assembly/disassembly on the biosensor surface, the large protein complexes are released from the surface for visualization and identification of components of the complexes using electron microscopy and mass spectrometry, respectively.

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Protein ComplexesBiolayer InterferometryMass SpectrometryElectron MicroscopyAnthrax ToxinBiosensorProtein AssemblyPH-induced Structural RearrangementsLFnNHSEDCPDEAL-cysteine

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Cite this Article

Machen, A. J., O'Neil, P. T., Pentelute, B. L., Villar, M. T., Artigues, A., Fisher, M. T. Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy. J. Vis. Exp. (138), e57902, doi:10.3791/57902 (2018).

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