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En enkel, rask og kvantitative analysen mål reparasjon av DNA-protein krysskoblinger på plasmider transfekterte i pattedyrceller
JoVE Journal
Genetica
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JoVE Journal Genetica
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

En enkel, rask og kvantitative analysen mål reparasjon av DNA-protein krysskoblinger på plasmider transfekterte i pattedyrceller

7,914 Views

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11:58 min

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March 05, 2018

DOI:

10.3791/57413-v

DOI:
10.3791/57413-v

•

11:58 min
March 05, 2018

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7 Views
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1Department of Pharmacology,University of Minnesota

Tags

DNA-protein CrosslinksPlasmid DNAMammalian CellsDNA RepairTrans-Pacific Primer Extension QPCR AssayAdductsPolymerase BlockingHouse Cell Reactivation8-oxoguanineT4 Polynucleotide KinasePrimer ExtensionPhenol-chloroform Extraction

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Chesner, L. N., Campbell, C. A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells. J. Vis. Exp. (133), e57413, doi:10.3791/57413 (2018).

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