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带谱系特异标记的间充质细胞脂肪分化电位的可视化与量化
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March 31, 2018
DOI:
1School of Biological Sciences,The University of Auckland, 2Research School of Biology,The Australian National University, 3Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences,The University of Auckland, 4Department of Plastic Surgery,Counties Manukau District Health Board, 5Department of Surgery, Faculty of Medical and Health Sciences,The University of Auckland, 6Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences,The University of Auckland, 7Maurice Wilkins Centre,The University of Auckland
Summary
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传统的评价脂肪分化的方法既便宜又容易使用, 但并不特定于基因表达的变化。我们已经开发出一种用谱系特异标记将间充质细胞分化为成熟脂肪的实验。这种检测方法在基础研究和临床医学中有不同的应用。
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Cite this Article
Eom, J., Feisst, V., Ranjard, L., Loomes, K., Damani, T., Jackson-Patel, V., Locke, M., Sheppard, H., Narayan, P., Dunbar, P. R. Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker. J. Vis. Exp. (133), e57153, doi:10.3791/57153 (2018).
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