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Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane

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08:32 min

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April 12, 2018

DOI:

10.3791/57084-v

DOI:
10.3791/57084-v

•

08:32 min
April 12, 2018

•

1 Views
, , , ,
1Department of Ophthalmology and Visual Science,Yale School of Medicine, 2Edward S. Harkness Eye Institute,Columbia University School of Medicine

Summary

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The goal of this protocol is to demonstrate the culturing of retinal pigment epithelial (RPE) cells on aged and/or diseased human Bruch's membrane. This method is suitable to study RPE cell behavior on a compromised extracellular matrix.

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Retinal Pigment Epithelial CellsBruch’s MembraneEx Vivo ModelAged HumanAMD EtiologyProteomicsRPE Cell CultureScleraVitreousRetinaOptic NerveAmmonium HydroxideDPBSPTFE Membrane

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Cite this Article

Cai, H., Gong, J., Del Priore, L. V., Tezel, T. H., Fields, M. A. Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane. J. Vis. Exp. (134), e57084, doi:10.3791/57084 (2018).

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