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Vurdering af den cytotoksiske og immunmodulerende virkninger af stoffer i menneskelige præcision-cut lunge skiver
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1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery,Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology,RWTH Aachen University, 6Institute for Immunology,Hannover Medical School
Capitoli
- 00:05Titolo
- 01:26General Preparation of Human PCLS and Subsequent Exposure to Chemicals
- 04:28Measurement of Chemically-induced Cytotoxicity in Human PCLS by WST-1 Assay
- 06:01LDH Assay
- 07:29Microscopic Viability Assay with Confocal Laser Scanning Microscope (cLSM)
- 09:49Results: Representative Images of the Three-dimensional Microscopic Staining and Image Rendering of Human PCLS
- 10:59Conclusion
I betragtning af 3R-princippet, respiratorisk modeller som alternativer til dyreforsøg er under udvikling. Især for risikovurdering af respiratorisk stoffer er der en mangel på passende assays. Her, beskriver vi brugen af menneskelige præcision-cut lunge skiver til vurdering af luftbårne stoffer.
