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转录因子结合、转录、翻译和营业额的实时分析在细胞激活过程中显示全球事件
JoVE Journal
Genetica
This content is Free Access.
JoVE Journal Genetica
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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转录因子结合、转录、翻译和营业额的实时分析在细胞激活过程中显示全球事件

12,982 Views

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12:54 min

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March 07, 2018

DOI:

10.3791/56752-v

DOI:
10.3791/56752-v

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12:54 min
March 07, 2018

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1Institute for Diabetes and Obesity (IDO), German Center for Diabetes Research (DZD),Helmholtz Zentrum München, 2Institute for Informatics,Ludwig-Maximilians-Universität München, 3Roche Pharma Research and Early Development, Large Molecule Research,Roche Innovation Center Penzberg

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Real-time AnalysisTranscription Factor BindingTranscriptionTranslationTurnoverCellular ActivationRNA RegulationDNA RegulationMolecular MechanismsImmune ResponseDrug Treatment4sU LabelingNewly Transcribed RNABiotinylationStreptavidin Beads

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Davari, K., Lichti, J., Friedel, C. C., Glasmacher, E. Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation. J. Vis. Exp. (133), e56752, doi:10.3791/56752 (2018).

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