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Live beelden van GLUT4 eiwit mensenhandel muis primaire hypothalame neuronen met behulp van deconvolutie microscopie
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1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Capitoli
- 00:05Titolo
- 01:00Primary Neuron Culture and Brain Tissue Isolation
- 02:56Tissue Digestion, Plating, and Culture
- 04:32Live Image Recording
- 06:48Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
- 07:47Conclusion
Dit protocol wordt een techniek beschreven voor observatie van de real-time groen fluorescentie proteïne (GFP) gelabeld Glucose Transporter 4 (GLUT4) eiwit mensenhandel op insuline stimulatie en karakterisering van de biologische rol van CCR5 in de insuline-GLUT4 signalering traject met deconvolutie microscopie.
