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用反褶积显微镜观察小鼠原发性下丘脑神经元 GLUT4 蛋白的动态图像

JoVE Journal
Neuroscienze

This content is Free Access.

JoVE Journal Neuroscienze

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

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用反褶积显微镜观察小鼠原发性下丘脑神经元 GLUT4 蛋白的动态图像

9,213 Views

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08:47 min

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December 07, 2017

DOI:

10.3791/56409-v

DOI:

10.3791/56409-v

•

08:47 min

December 07, 2017

•

3 Views

Chun Austin Changou^1,2,3, Reni Ajoy^4,5, Szu-Yi Chou^4,5,6

¹The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, ²Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, ³Core Facility,Taipei Medical University, ⁴The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, ⁵Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, ⁶TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University

Summary

本协议描述了一种实时绿色荧光蛋白 (GFP) 标记葡萄糖转运蛋白 4 (GLUT4) 蛋白质贩运的技术, 对胰岛素刺激和 CCR5 在 insulin–GLUT4 的生物学作用的表征带反褶积显微镜的信号通路。

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