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Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
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Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
1BIOS Lab on a Chip group, MIRA Institute for Biomedical Technology and Technical Medicine, MESA+ Institute for Nanotechnology and Max Planck Center for Complex Fluid Dynamics,University of Twente, 2Microsystems,Eindhoven University of Technology, 3Applied Stem Cell Technologies, MIRA Institute for Biomedical Technology and Technical Medicine,University of Twente
Capitoli
- 00:05Titolo
- 01:11Fabrication of the Organ-on-chip Device
- 05:37On-chip Culture of Brain-derived Endothelial Cells
- 08:42Results: Representative Impedence Data, TEER Determination and Development of BBB-on-chip
- 10:04Conclusion
This publication describes the fabrication of an organ-on-chip device with integrated electrodes for direct quantification of transendothelial electrical resistance (TEER). For validation, the blood-brain barrier was mimicked inside this microfluidic device and its barrier function was monitored. The presented methods for electrode integration and direct TEER quantification are generally applicable.
