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Etablering av larver sebrafisk som et dyr-modellen for å undersøke Trypanosoma cruzi motilitet I Vivo
JoVE Journal
Biologia dello sviluppo
Author Produced
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
DOI:

13:21 min

September 30, 2017

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Capitoli

  • 00:00Titolo
  • 00:13Introduction
  • 03:09Obtaining Embryos for Injection
  • 04:13Preparing Injection Material
  • 05:38Obtaining Labeled T. cruzi Parasites for Injection
  • 07:04Injecting Zebrafish Larvae
  • 08:10LSFM Mounting and Imaging of Injected Larvae
  • 08:57LSFM Imaging of Injected Larvae
  • 10:44Representative Results
  • 11:40Conclusion

Summary

Traduzione automatica

I denne protokollen, fluorescently merket T. cruzi ble injisert i gjennomsiktig sebrafisk larver og parasitten motilitet ble observert i vivo bruker lys ark fluorescens mikroskopi.

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