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低丰度胚胎标本的染色质沉淀 (芯片) 协议
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Genetica
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JoVE Journal Genetica
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
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低丰度胚胎标本的染色质沉淀 (芯片) 协议

DOI:
10.3791/56186-v

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12:47 min

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August 29, 2017

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, , , ,
1Center for Molecular Medicine Cologne (CMMC),University of Cologne, 2Cologne Center for Genomics (CCG),University of Cologne, 3Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Research Center and Systems Biology of Ageing Cologne,University of Cologne

Capitoli

  • 00:05Titolo
  • 00:57Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)
  • 04:12Crosslinking Proteins to DNA
  • 05:01Lysis, Sonication, and Immunoprecipitation
  • 06:33Washing, Elution, and Reversal of Crosslinking
  • 08:57Cellular Protein and RNA Digestion
  • 10:06Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments
  • 11:48Conclusion

Summary

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在这里, 我们描述了染色质沉淀 (芯片) 和芯片序列库的准备协议, 以产生全球表剖面从丰鸡胚胎标本。

Tags

Chromatin Immunoprecipitation (ChIP)Low-abundance Embryonic SamplesHistone ModificationsVertebrate GenomesBiologia dello sviluppoEnhancersGenesTranscription RegulationCellular HeterogeneityPatient SamplesChicken EmbryosHH19Extra-embryonic MembranesSpinal Neuro Tube (SNT)TrypsinizationDMEMFBS

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