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水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

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水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案

DOI:

10.3791/56106-v

•

08:31 min

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August 02, 2017

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Lucia Salz², Ryan R. Driskell²

¹Centre for Stem Cells and Regenerative Medicine,King’s College London, ²School of Molecular Biosciences,Washington State University

Capitoli

00:05Titolo
00:55Preparation of Skin Samples
02:07Cryosectioning
03:17Immunofluorescent Labeling
05:05Mounting for Microscopic Visualization
06:36Results: Horizontal Whole Mount Compared to a Classical Cryosection
07:55Conclusion

Summary

Traduzione automatica

这项工作提出了一种新颖的加工和成像协议，用于厚的三维组织横截面分析，可以充分利用共聚焦成像模式。该协议保留抗原性，并代表一个强大的系统来分析皮肤组织学和潜在的其他组织类型。

Tags

Keywords: Horizontal Whole Mount Skin Imaging Protocol Confocal Microscopy Cryostat Sectioning Immunofluorescent Labeling

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