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自动图像引导贴片夹在脑切片神经元研究中的应用
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Neuroscienze
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JoVE Journal Neuroscienze
Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
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自动图像引导贴片夹在脑切片神经元研究中的应用

DOI:
10.3791/56010-v

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09:05 min

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July 31, 2017

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,
1Department of Biological Sciences,Purdue University, 2Purdue Institute for Integrative Neuroscience,Purdue University

Capitoli

  • 00:05Titolo
  • 01:16Run Autopatcher IG
  • 02:48Automatic Patch Clamp Procedure
  • 07:03Results: Conducting an Automated Drug Delivery Experiment
  • 08:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了如何使用最近为标准体外电生理设备开发的系统进行自动图像引导的膜片钳实验。

Tags

Keywords: Automated Image-guided Patch ClampNeuronsBrain SlicesComputer VisionFluorescent CellsPatch Clamp ElectrophysiologyHigh ThroughputNeurological DisordersPharmacological ScreeningNeuronal PreparationsBrain SliceSlice CultureDissociated NeuronsCalibrationManipulatorMicroscopeAmplifierAutopatcher IGInternal SolutionPipette TipMicroscope StageCoordinate System

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