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Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
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JoVE Journal Neuroscienze
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction

DOI:
10.3791/55994-v

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08:11 min

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August 25, 2017

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, , , ,
1Department of Pharmacology, Toxicology, and Neuroscience,Louisiana State University Health Sciences Center, 2Department of Molecular & Integrative Physiology,University of Kansas Medical Center

Capitoli

  • 00:05Titolo
  • 00:58Preparation for Injection
  • 02:47Vector Injection
  • 04:55Quantification of GFP Expression in the Cerebellum
  • 06:48Rapid Quantification of the GFP-positive Fluorescent Area in the Cerebellum After Intravenous AAV Administration to the Rat
  • 07:30Conclusion

Summary

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Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.

Tags

Keywords: Intravenous AdministrationAdeno-associated VirusRatsCentral Nervous System TransductionGene TransferAmyotrophic Lateral SclerosisSpinal CordAnesthesiaTail Vein Injection

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