Rivista
/
Biologia dello sviluppo
/
In Ovo Electroporation in the Chicken Auditory Brainstem
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
In Ovo Electroporation in the Chicken Auditory Brainstem

In Ovo Electroporation in the Chicken Auditory Brainstem

8,291 Views

•

10:14 min

•

June 09, 2017

DOI:

10.3791/55628-v

DOI:
10.3791/55628-v

•

10:14 min
June 09, 2017

•

1 Views
, ,
1Roxelyn and Richard Pepper Department of Communication Sciences and Disorders,Northwestern University, 2Knowles Hearing Research Center,Northwestern University, 3Department of Neurobiology,Northwestern University

Summary

Automatically generated

Auditory brainstem neurons of avians and mammals are specialized for fast neural encoding, a fundamental process for normal hearing functions. These neurons arise from distinct precursors of embryonic hindbrain. We present techniques utilizing electroporation to express genes in the hindbrain of chicken embryos to study gene function during auditory development.

Explore More Videos

In Ovo ElectroporationChicken Auditory BrainstemGene ExpressionAuditory Neuron DevelopmentPlasmid SolutionMicropipette PullerPicospritzerHamburger-Hamilton StageEgg IncubationEggshell Membrane RemovalAlbumin Withdrawal

Read Article

Cite this Article

Lu, T., Cohen, A. L., Sanchez, J. T. In Ovo Electroporation in the Chicken Auditory Brainstem. J. Vis. Exp. (124), e55628, doi:10.3791/55628 (2017).

Download .ris file

Copy

Share Video

.

Copy