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全细胞电压钳记录的电生理方法<em>果蝇</em>光感受器
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors
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全细胞电压钳记录的电生理方法<em>果蝇</em>光感受器

DOI:
10.3791/55627-v

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10:36 min

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June 13, 2017

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, , , ,
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC),Hebrew University, 2Department of Physiology, Development and Neuroscience,University of Cambridge

Capitoli

  • 00:05Titolo
  • 01:01Retina Dissection and Ommatidia Isolation
  • 05:32Whole-cell Recording
  • 08:21Results: The inaCP209 and inaDP215 Mutants Reveal Slow Respomse Termination of the Microscopic Response to Light and of the Single Quantum Bumps
  • 09:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

从黑腹果蝇光感受器的全细胞记录使得能够测量自发的黑色凸起，量子隆起，对光的宏观反应以及在各种条件下的电流 - 电压关系。与黑腹果蝇遗传操作工具结合使用，该方法能够研究普遍存在的肌醇脂质信号通路及其靶标TRP通道。

Tags

Keywords: ElectrophysiologyWhole-cell Voltage ClampDrosophilaPhotoreceptorsPhototransductionTRP ChannelsVisual TransductionDissectionCalcium-free Extracellular SolutionInverted MicroscopePerfusion SystemSuction SystemGround WireElectrophysiology Setup

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