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High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
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JoVE Journal Biochimica
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

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11:24 min

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May 13, 2017

DOI:

10.3791/55623-v

DOI:
10.3791/55623-v

•

11:24 min
May 13, 2017

•

3 Views
, , , , ,
1Department of Chemistry,Clemson University, 2Department of Physics and Astronomy,Clemson University, 3Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences,University of Texas Health Science Center

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High Precision FRETSingle-moleculeBiomolecule Structure DeterminationFörster Resonance Energy TransferMultiparameter Fluorescence DetectionInterdye Distance MeasurementNMDA ReceptorConformational StatesStructural ModelDynamic Biomolecules

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Ma, J., Yanez-Orozco, I. S., Rezaei Adariani, S., Dolino, D., Jayaraman, V., Sanabria, H. High Precision FRET at Single-molecule Level for Biomolecule Structure Determination. J. Vis. Exp. (123), e55623, doi:10.3791/55623 (2017).

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