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IL-8瞬时转化的小鼠模型<em>体内</em>长期监测炎症反应
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July 07, 2017
DOI:
1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory “D. Lissandrini”,University of Verona, 2Corporate Preclinical R&D,Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division,University of Verona, 4Dipartimento di Scienze Medico-Veterinarie,University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences,University of Parma, 6Department of Computer Science,University of Verona, 7Department of Neurological, Biomedical and Movement Sciences,University of Verona, 8Cystic Fibrosis Regional Center (CFC),AOUI Verona
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这里描述的方法允许通过非侵入性生物发光成像（BLI）在小鼠肺中IL-8启动子依赖性炎症激活的可视化。荧光素酶报告基因构建体的交付时间可以将相同的动物多次进行BLI多至两个月。
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Bergamini, G., Stellari, F., Sandri, A., M. Lleo, M., Donofrio, G., Ruscitti, F., Boschi, F., Sbarbati, A., Villetti, G., Melotti, P., Sorio, C. An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses. J. Vis. Exp. (125), e55499, doi:10.3791/55499 (2017).
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