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基于核糖体 dna 的 RNA 样本 dna 污染评价
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January 22, 2018
DOI:
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT),Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology,National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology,Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Summary
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在这里, 我们提出了一个在 RNA 样本中追踪基因组 DNA (gDNA) 污染的协议。所提出的方法利用特定的引物的内部转录间隔区 (其) 的核糖体 DNA (rDNA) 基因。该方法适用于大多数真核生物和原中 DNA 污染的可靠和灵敏检测。
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Cite this Article
Hashemipetroudi, S. H., Nematzadeh, G., Ahmadian, G., Yamchi, A., Kuhlmann, M. Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA. J. Vis. Exp. (131), e55451, doi:10.3791/55451 (2018).
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