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Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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JoVE Journal Medicina
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

DOI:
10.3791/55147-v

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10:35 min

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December 03, 2017

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, , , , , , ,
1Institute of Medical Microbiology, Immunology and Parasitology,University Hospital Bonn, 2Department of Orthopaedics and Trauma Surgery,University Hospital Bonn, 3Division of EU Cooperation/Microbiology,Paul-Ehrlich-Institute

Capitoli

  • 00:05Titolo
  • 00:53Joint Aspiration
  • 03:19PCR Diagnostics
  • 05:34Sonication
  • 07:57Results: Implant Sonication and Multiplex PCR as Diagnostics in Revision Arthroplasty
  • 09:36Conclusion

Summary

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Traduzione automatica

Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.

Tags

Keywords: Revision ArthroplastyImplant SonicationMultiplex Polymerase Chain ReactionPeri-prosthetic Joint InfectionJoint AspirationMicrobiological ResultsPathogen DetectionDifferential DiagnosticsJoint Fluid AspirationBlood Culture FlaskPCR AnalysisCell CountEDTA

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