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兹卡病毒感染细胞培养系统和<em>体外</em>干扰素的预防作用
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons
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兹卡病毒感染细胞培养系统和<em>体外</em>干扰素的预防作用

20,465 Views

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09:11 min

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August 23, 2016

DOI:

10.3791/54767-v

DOI:
10.3791/54767-v

•

09:11 min
August 23, 2016

•

4 Views
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1Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 2Department of Surgery,Cedars-Sinai Medical Center, 3Department of Surgery, David Geffen School of Medicine,University of California, Los Angeles

Summary

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Zika Virus (ZIKV), an emerging pathogen, is linked to fetal developmental abnormalities and microcephaly. The establishment of an effective infectious cell culture system is crucial for studies of ZIKV replication as well as vaccine and drug development. In this study, various virological assays pertaining to ZIKV are illustrated and discussed.

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Zika VirusInfectious Cell Culture SystemIn VitroProphylactic EffectInterferonsVero CellsViral InoculumViral PlaqueCell Culture SupernatantDrug DevelopmentVaccine Development

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Cite this Article

Contreras, D., Arumugaswami, V. Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons. J. Vis. Exp. (114), e54767, doi:10.3791/54767 (2016).

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