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在拟南芥原生质体转染昼夜的表型有荧光时钟记者的快速分析
JoVE Journal
Biologia
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JoVE Journal Biologia
Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
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在拟南芥原生质体转染昼夜的表型有荧光时钟记者的快速分析

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07:42 min

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September 17, 2016

DOI:

10.3791/54586-v

DOI:
10.3791/54586-v

•

07:42 min
September 17, 2016

•

3 Views
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1Institute for Molecular Plant Sciences,University of Edinburgh

Summary

Automatically generated

生物钟的调节有关拟南芥转录组的三分之一，但基因的反馈到报时的比例尚不清楚。在这里，我们可视化的方法来使用一昼夜记者在原生质体瞬时表达的荧光成像拟南芥突变体的任何行快速评估昼夜的表型。

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Cite this Article

Hansen, L. L., van Ooijen, G. Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter. J. Vis. Exp. (115), e54586, doi:10.3791/54586 (2016).

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