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単離、分化、および血液からヒト抗体を分泌するB細胞の定量化：体液性免疫の機能読み出しなどのELISpot
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
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単離、分化、および血液からヒト抗体を分泌するB細胞の定量化：体液性免疫の機能読み出しなどのELISpot

14,857 Views

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08:26 min

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December 14, 2016

DOI:

10.3791/54582-v

DOI:
10.3791/54582-v

•

08:26 min
December 14, 2016

•

7 Views
1Graduate Institute of Pharmacology, College of Medicine,National Taiwan University

Summary

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Human peripheral blood is commonly used for the assessment of the humoral immune response. Here, the methods for isolating human B cells from peripheral blood, differentiating human B cells into antibody (Ab)-secreting B cells (ASCs) in culture, and enumerating the total IgM- and IgG-ASCs via an ELISpot assay are described.

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Tzeng, S. The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity. J. Vis. Exp. (118), e54582, doi:10.3791/54582 (2016).

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