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적용 형광 공명 에너지 전달 (FRET)을하여 이펙터 전좌 효율을 검사합니다<em> Coxiella burnetii의</em> siRNA를 음소거시
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JoVE Journal Immunologia e infezioni
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
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적용 형광 공명 에너지 전달 (FRET)을하여 이펙터 전좌 효율을 검사합니다<em> Coxiella burnetii의</em> siRNA를 음소거시

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10:29 min

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July 06, 2016

DOI:

10.3791/54210-v

DOI:
10.3791/54210-v

•

10:29 min
July 06, 2016

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1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,University of Melbourne

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FRETEffector TranslocationSiRNA SilencingCoxiella BurnetiiCellular MicrobiologyHost-pathogen InteractionsChlamydiaSalmonellaE. ColiBeta-lactamaseACCM2HeLa CellsTransfection96-well Plate

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Newton, P., Latomanski, E. A., Newton, H. J. Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing. J. Vis. Exp. (113), e54210, doi:10.3791/54210 (2016).

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