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によってエフェクター転効率を検査するための蛍光共鳴エネルギー移動（FRET）を適用<em> Q熱リケッチア</em>のsiRNAサイレンシング中
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
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によってエフェクター転効率を検査するための蛍光共鳴エネルギー移動（FRET）を適用<em> Q熱リケッチア</em>のsiRNAサイレンシング中

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10:29 min

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July 06, 2016

DOI:

10.3791/54210-v

DOI:
10.3791/54210-v

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10:29 min
July 06, 2016

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1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,University of Melbourne

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FRETEffector TranslocationSiRNA SilencingCoxiella BurnetiiCellular MicrobiologyHost-pathogen InteractionsChlamydiaSalmonellaE. ColiBeta-lactamaseACCM2HeLa CellsTransfection96-well Plate

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Newton, P., Latomanski, E. A., Newton, H. J. Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing. J. Vis. Exp. (113), e54210, doi:10.3791/54210 (2016).

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